Evaluation on antioxidative capacity, nephroprotective effect, and DNA damage protection of mixed low potassium vegetables and fruits juice powder in HEK-293 cells

Matthawan Khamhae, Kanyanatt Kanokwiroon, Worrapanit Chansuwan, Nualpun Sirinupong

Abstract


Background: An imbalance between free radical production and antioxidant defenses is one of the multifactorial natures contributing to many disorders. One of them is chronic kidney disease (CKD). The development of novel diets to slow progression or reduces complication of CKD are highly needed. Therefore, the objective of this study was to evaluate the in vitro antioxidative capacity and nephroprotective effect of a product, mixed low potassium vegetables and fruits juice powder (MJP) in HEK-293 cells.

Methods: The produced MJP was determined for phenolics and flavonoids content. The antioxidative capacity was examined by DPPH, FRAP and ABTS assay. The cell viability to MJP and hydrogen peroxide (H2O2)-treated were determined by MTT assay. The intracellular antioxidative capacity was determined by co-treatment of MJP and H2O2-induced HEK-293 cell damage including reactive oxygen species (ROS) production, intracellular enzymes activities, and DNA damage protection.

Results: The MJP at the concentration of 5 and 10 mg/ml showed positive effects on H2O2-induced HEK-293 cells protection and significantly increased cell viability. The ROS production measured by 2’,7’-dichlorodihydrofluorescein diacetate (DCF) were increased in the H2O2 treatment approximately 2 times but significantly decreased in the MJP treatment. The result of intracellular enzymatic antioxidant markers, including superoxide dismutase (SOD) and catalase (CAT) activities were increased while the Malonaldehyde (MDA) level was attenuated in the co-treatment of MJP and H2O2-induced HEK293 cells compared with only H2O2-inducing. In the examination of the morphological change due to oxidative stress exhibited less nuclei fragment in the cell of MJP treatment. In addition, the isolated DNA was protected by MJP application.   

Conclusion: The produced MJP contains bioactive phenolic and flavonoid compounds. MJP possesses potential antioxidative capacity through the reducing H2O2-induced HEK-293 cells damage and increasing the intracellular antioxidant enzymes. In addition, the nephroprotective effects of MJP related to the antioxidant enzymes was involved in the free radical scavenging. Therefore, MJP could be a functional drink for individual limiting potassium and liquid uptake.

Keywords:  Antioxidative capacity, DNA damage protection, low potassium vegetables and fruits, nephroprotective, chronic kidney disease 

 


References


Corpas F.J, Barroso J.B. 2013. Nitro‐oxidative stress vs oxidative or nitrosative stress in higher plants. New Phytologist, 199(3), 633-635. http:/doi: 10.1111/nph.12380

Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della M.D. et al. 2018. Oxidative stress, aging, and diseases. Clinical interventions in aging, 13, 757-772. http://doi: 10.2147/CIA.S158513

Biswas S.K. 2016. Does the interdependence between oxidative stress and inflammation explain the antioxidant paradox?. Oxidative Medicine and Cellular Longevity, 2016. http://doi: 10.1155/2016/5698931

Sundaram S. P. M, Nagarajan S, Devi A.J.M. 2014. Chronic Kidney Disease—Effect of Oxidative Stress. Chinese Journal of Biology, 2014. http://doi: 10.1155/2014/216210

Sung C.C, Hsu Y.C, Chen C.C, Lin Y.F, Wu C.C. 2013. Oxidative stress and nucleic acid oxidation in patients with chronic kidney disease. Oxidative Medicine and Cellular Longevity, 2013. https://doi.org/10.1155/2013/301982

Boeing H, Bechthold A, Bub A, Ellinger S, Haller D, Kroke A, et al. 2012. Critical review: vegetables and fruit in the prevention of chronic diseases. European journal of nutrition, 51(6), 637-663. http://doi: 10.1007/s00394-012-0380-y

Jun M, Venkataraman V, Razavian M, Cooper B, Zoungas S, Ninomiya T, et al. 2012. Antioxidants for chronic kidney disease. The Cochrane database of systematic reviews, 10, Cd008176. doi: 10.1002/14651858.CD008176.pub2

Marques de Mattos A, Afonso Jordao A, Abrao Cardeal da Costa J, Garcia Chiarello P. 2014. Study of Protein Oxidative Stress, Antioxidant Vitamins and Inflammation in Patients Undergoing either Hemodialysis or Peritoneal Dialysis. Int J Vitam Nutr Res, 84(5-6), 261-268. http://doi: 10.1024/0300-9831/a000212

Chen J, Siriki R. 2015. Antioxidants Therapy for Patients with Chronic Kidney Disease: A Question of Balance. American Journal of Nephrology, 42(4), 318-319. http://doi: 10.1159/000441628

Cai Y, Luo Q, Sun M, Corke H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci, 74(17), 2157-2184. http://doi: 10.1016/j.lfs.2003.09.047

Zhang L, Ravipati A.S, Koyyalamudi S.R, Jeong S.C, Reddy N, Smith P.T, Wu M.J. 2011. Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds. Journal of agricultural and food chemistry, 59(23), 12361-12367.

Fu L, Xu B.T, Xu X.R, Gan R.Y, Zhang Y, Xia E.Q, Li H.B. 2011. Antioxidant capacities and total phenolic contents of 62 fruits. Food Chemistry, 129(2), 345-350. http://doi: 10.1016/j.foodchem.2011.04.079

Lutz M, Hernández J, Henríquez C. 2015. Phenolic content and antioxidant capacity in fresh and dry fruits and vegetables grown in Chile. CyTA - Journal of Food, 13(4), 541-547. http://doi: 10.1080/19476337.2015.1012743

Álvarez R, Araya H, Navarro-Lisboa R, Lopez de Dicastillo C. 2016. Evaluation of Polyphenol Content and Antioxidant Capacity of Fruits and Vegetables Using a Modified Enzymatic Extraction. Food technology and biotechnology, 54(4), 462-467. http://doi: 10.17113/ftb.54.04.16.4497

Fu L, Xu B.T, Xu X.R, Qin X.S, Gan R.Y, Li H.B. 2010. Antioxidant capacities and total phenolic contents of 56 wild fruits from South China. Molecules, 15(12), 8602-8617. http://doi: 10.3390/molecules15128602

Tinrat S. 2016. Antioxidant activities and total phenolic content of multi-colored fruits and vegetables in Thailand. KKU Research Journal, 21(1), 1-11. http://doi: 10.14456/kkurj.2016.1

De Nicola L, Zoccali, C. 2016. Chronic kidney disease prevalence in the general population: heterogeneity and concerns. Nephrol Dial Transplant, 31(3), 331-335. http://doi: 10.1093/ndt/gfv427

Vejakama P, Insathit A, Thakkinstian A. 2015. Chronic kidney disease prevalence in Thailand. [http://kb.hsri.or.th/dspace/handle/11228/4345] Retrieved December 31, 2019.

Ash S, Campbell K, MacLaughlin H, McCoy E, Chan M, Anderson K, et al. 2006. Evidence based practice guidelines for the nutritional management of chronic kidney disease. Nutrition & Dietetics, 63, S33-S45.

Sousa B.A, Correia R.T.P. 2012. Phenolic content, antioxidant activity and antiamylolytic activity of extracts obtained from bioprocessed pineapple and guava wastes. Brazilian Journal of Chemical Engineering, 29(1), 25-30.

Rebaya A, Belghith S.I, Baghdikian B, Leddet V.M, Mabrouki F, Olivier E, et al. 2014. Total phenolic, total flavonoid, tannin content, and antioxidant capacity of Halimium halimifolium (Cistaceae). Journal of applied pharmaceutical science, 5(1), 52-57.

Pyo Y. H, Jin Y.J, Hwang J.Y. 2014. Comparison of the effects of blending and juicing on the phytochemicals contents and antioxidant capacity of typical Korean kernel fruit juices. Preventive nutrition and food science, 19(2), 108.

Young I, Woodside J. 2001. Antioxidants in health and disease. Journal of clinical pathology, 54(3), 176-186.

Arnao M. B, Cano A, Acosta M. 2001. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chemistry, 73(2), 239-244.

Athmouni K, Belhaj D, El Feki A, Ayadi H. 2018. Optimization, antioxidant potential, modulatory effect and anti-apoptotic action in of Euphorbia bivonae polysaccharides on hydrogen peroxide-induced toxicity in human embryonic kidney cells HEK293. International journal of biological macromolecules, 116, 482-491.

Reshma A.K.P, Brindha P. 2014. In vitro anti-inflammatory, antioxidant and nephroprotective studies on leaves of Aegle marmelos and Ocimum sanctum. Asian J Pharm Clin Res.

Kalaivani M.K, Soundararajan P, Vasanthi H.R, Sumathy A. 2015. In-vitro Nephroprotective role of Ethanolic root extract of Boerhaavia diffusa against cisplatin induced nephrotoxicity. International Journal of Phytomedicine.

Elumalai P, Gunadharini D, Senthilkumar K, Banudevi S, Arunkumar R, Benson C, et al. 2012. Induction of apoptosis in human breast cancer cells by nimbolide through extrinsic and intrinsic pathway. Toxicology letters, 215(2), 131-142.

Bian Y. Y, Guo J, Majeed H, Zhu K. X, Guo X. N, Peng W, et al. 2015. Ferulic acid renders protection to HEK293 cells against oxidative damage and apoptosis induced by hydrogen peroxide. In Vitro Cellular & Developmental Biology-Animal, 51(7), 722-729.

Anantachoke N, Lomarat P, Praserttirachai W, Khammanit R, Mangmool S. 2016. Thai fruits exhibit antioxidant activity and induction of antioxidant enzymes in HEK-293 cells. Evidence-Based Complementary and Alternative Medicine.

Jiang Y, Han W, Shen T, Wang M.H. 2012. Antioxidant activity and protection from dna damage by water extract from pine (Pinus densiflora) bark. Preventive nutrition and food science, 17(2), 116.

Pachotikarn C. 2016. Management of food intake in dialysis patient. Thai dietetics. [http://www.thaidietetics.org/File/Share_Learn/SL20120604032110_Mgt%20of%20Food%20intake%20(Chanida%20Pachotikarn%20).pdf] Retrieved December 31, 2019.

Nti C.A, Hagan J, Bagina F, Seglah M. 2011. Knowledge of nutrition and health benefits and frequency of consumption of fruits and vegetables among Ghanaian homemakers. African Journal of Food Science, 5(6), 333-339.

Guo X, Li T, Tang K, Liu, R.H. 2012. Effect of Germination on Phytochemical Profiles and Antioxidant Activity of Mung Bean Sprouts (Vigna radiata). Journal of Agricultural and Food Chemistry, 60(44), 11050-11055. http://doi: 10.1021/jf304443u

Kapusta-Duch J, Kopec A, Piatkowska E, Borczak B, Leszczynska T. 2012. The beneficial effects of Brassica vegetables on human health. Roczniki Państwowego Zakładu Higieny, 63(4).

Naz A, Butt M.S, Sultan M.T, Qayyum M.M.N, Niaz R.S. 2014. Watermelon lycopene and allied health claims. EXCLI journal, 13, 650-660.

Endrizzi I, Torri L, Corollaro M.L, Demattè M.L, Aprea E, Charles M, et al. 2015. A conjoint study on apple acceptability: Sensory characteristics and nutritional information. Food quality and preference, 40, 39-48.

Hossain M.F, Akhtar S, Anwar M. 2015. Nutritional value and medicinal benefits of pineapple. International Journal of Nutrition and Food Sciences, 4(1), 84-88.

Sun X, Baldwin E.A, Plotto A, Manthey J.A, Duan Y, Bai J. 2017. Effects of thermal processing and pulp filtration on physical, chemical and sensory properties of winter melon juice. Journal of the Science of Food and Agriculture, 97(2), 543-550.

WHO. 2003. Diet, nutrition, and the prevention of chronic diseases: report of a joint WHO/FAO expert consultation (Vol. 916): World Health Organization. [https://apps.who.int/iris/bitstream/handle/10665/42665/WHO_TRS_916.pdf;jsessionid=922B5DF9256628FB4E545CD9157DF596?sequence=1] Retrieved December 31, 2019.

WHO. 2003. Diet, nutrition, and the prevention of chronic diseases: report of a joint WHO/FAO expert consultation (Vol. 916): World Health Organization. [https://apps.who.int/iris/bitstream/handle/10665/42665/WHO_TRS_916.pdf;jsessionid=922B5DF9256628FB4E545CD9157DF596?sequence=1] Retrieved December 31, 2019.

Gülcin I. 2012. Antioxidant activity of food constituents: an overview. Archives of toxicology, 86(3), 345-391.

Jones D.P. 2006. Redefining oxidative stress. Antioxidants & redox signaling, 8(9-10), 1865-1879.

da Silva Pitz H, Pereira A, Voytena A.P.L, Trevisan A.C.D, Affonso R.C.L, Ribeiro-do-Valle R.M, Maraschin M. 2017. Cytoprotective Effects of Jaboticaba (Pliniaperuviana, Poir. Govaerts) Fruit Peel Extracts against H2O2-Induced Oxidative Stress. Reactive Oxygen Species, 4(11), 362-371.

Pandareesh M.D, Anand T, Bhat P.V. 2016. Cytoprotective propensity of Bacopa monniera against hydrogen peroxide induced oxidative damage in neuronal and lung epithelial cells. Cytotechnology, 68(1), 157-172.


Full Text: [Abstract] [Full Article]

DOI: 10.31989/ffhd.v10i3.698

Refbacks

  • There are currently no refbacks.




Copyright (c) 2020 Nualpun Sirinupong

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
x
Message