Integrated Medicine with vegetable derived antioxidant and vitamin D : effects on oxidative stress and bone mineral metabolism of aged patients with renal disease


  • Francesco Carluccio
  • Macello Lenucci
  • Gabriella Piro
  • Werner Siems
  • Josè Luño



Background: Aging and chronic kidney disease (CKD) are associated with bone mineral metabolism disorders, in addition to disarrangement of trabeculae structure and bone architecture. Increased alkaline phosphatase (ALP) and parathyroid hormone (PTH) have been related with abnormal bone turn over. Lycopene, a plant derived micronutrient, has strong quenching and free radical scavenging attitude.

Objectives: This study aimed to evaluate the effects of lycopene and calcifediol (25 OH D3) based Integrated Medicine on ALP, PTH and oxidative stress.

Methods: In octogenarians, nonagenarians and centenarians with chronic kidney disease (GFR :45 ±10,4 ml/min/1,73 m2), vitamin D deficiency and abnormal ALP, PTH blood values, the effects of daily lycopene supplementation on blood oxysterols and on 4-hydroxy-2,3-trans- nonenal (4-HNE) as markers of oxidative stress were evaluated. The effects of calcifediol administration together with daily lycopene supplementation on PTH and ALP blood concentrations were also investigated.

Results: Daily lycopene supplementation induced a reduction of oxysterols (α-triol: 0.40 ± 0.07 μg / L vs 0.32 ± 0, 04 μg / L and β-epoxi cholesterol: 5.3 ± 1.3 μg / L vs 3.7 ± 0.6 μg / L) but not of 4-HNE (0.27 ± 0.18 nmol / L vs 0.25 ± 0.20 nmol / L). Vitamin D added to lycopene for two weeks decreased the blood values of ALP (106 ± 40 U / L vs 69 ± 19 U / L) and PTH (108 ±42 pg/ml vs 66± 21 pg/ml).

Conclusion: Tomato derived lycopene, with daily supplements, decreased cholesterol oxidation products. Calcifediol and vegetable derived antioxidant daily supplementations were associated to normalization of ALP and PTH. 

Keywords: oxysterols; 4-hydroxynonenal; lycopene; alkaline phosphatase; parathyroid hormone; chronic kidney disease (CKD) 





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