Effect of ultradisperse humic sapropel suspension on collagenolytic enzyme activity of Coprinus lagopides mushroom culture in dynamics
DOI:
https://doi.org/10.31989/ffhd.v16i6.2043Abstract
Background: Collagenolytic enzymes, known as collagenases, are of significant interest in biotechnology, medicine, and the food industry due to their ability to break down collagen, the primary structural protein of connective tissue. In this study, the collagenolytic enzyme activity of a C. lagopides mushroom culture was assessed dynamically at various growth stages.
Objective: This study aims to determine the temporal profile of collagenase production to identify the most active phases of cultivation.
Methods: The C. lagopides culture grew under controlled conditions for 6 days at 25 °C. Collagenolytic activity was assessed photometrically. Samples were taken every 24 hours. The pH of the medium and the biomass of the fungal culture were also measured.
Results: The maximum collagenolytic activity occurred during the stationary phase of fungal growth. The addition of UDHSS significantly increased enzyme production, indicating a stimulatory effect on fungal metabolism. Experiments on the hydrolysis of native pollock skin collagen demonstrated a gradual increase in collagenolytic activity over time, with the highest activity observed after 6 hours of incubation.
Conclusion: The obtained results confirm the potential of Coprinus lagopides as a promising fungal producer of collagenolytic enzymes. The study also demonstrates the applicability of this enzymatic system for the biotechnological processing of collagen-containing fish raw materials and the production of collagen hydrolysates.
Novelty of the Study: The scientific novelty of this study lies in establishing patterns of change in the collagenolytic activity of Coprinus lagopides culture and identifying the stimulating effect of an ultradisperse humic sapropel suspension (UDHSS) on the activity of collagenolytic enzymes. For the first time, the potential application of the C. lagopides enzymatic system for the hydrolysis of fish-derived collagen was demonstrated.
Keywords: collagenolytic enzymes, bioactive compounds, fungal metabolism, functional food development, biochemical biomarkers, humic sapropel, enzymatic hydrolysis, time-dependent functional response.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Alina Sokolova, Nadezhda Barakova, Elena Kiprushkina, Vladimir Sadovoy, Olga Volkova, Daniel Nsengumuremyi, Arsenyeva Tamara, Khamitsaeva Alla
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain the copyright of their articles and grant the Functional Food Center (FFC) and its journals the right of first publication under the terms of the Creative Commons Attribution 4.0 International License.
This license permits unrestricted use, distribution, and reproduction in any medium, including commercial use, provided the original author(s) and source are properly credited. Authors may post and share their published work freely, provided that the original publication in this journal is acknowledged.
By submitting to this journal, authors confirm that their manuscripts are original, not under consideration elsewhere, and that they hold the necessary rights to grant this license. The Functional Food Center encourages open scientific exchange and allows derivative and extended works, provided attribution to the original publication is maintained.