9-cis β-carotene Inhibits Atherosclerosis Development in Female LDLR-/- Mice

Noa Zolberg Relevy, Ralph Rühl, Ayelet Harari, Itamar Grosskopf, Iris Barshack, Ami Ben-Amotz, Uri Nir, Hugo Gottlieb, Yehuda Kamari, Dror Harats, Aviv Shaish

Abstract


Background: Several epidemiological studies have shown that diets rich in carotenoids are associated with a reduced risk of cardiovascular disease. However, administration of synthetic all-trans b-carotene was reported to have no effect on cardiovascular disease. We previously showed that the 9-cis b-carotene-rich powder of the alga Dunaliella bardawil inhibits atherogenesis and reduces plasma non-HDL cholesterol levels in mice.

Context and purpose of this study: We sought to study whether isolated 9-cis b-carotene inhibits atherogenesis in a murine model of atherosclerosis.

Results: Twelve-week-old female LDL receptor knockout mice (LDLR-/-) were pretreated for  2 weeks with regular chow diet fortified with the alga Dunaliella powder, 9-cis β-carotene isomer, all-trans β-carotene isomer, or 9-cis retinoic acid, followed by 10 weeks of a high-fat diet with the same fortifications. In contrast to Dunaliella, 9-cis β-carotene did not inhibit the high fat diet-induced elevation of plasma cholesterol. In addition, diet fortification with Dunaliella powder, β-carotene isomers, or 9-cis retinoic acid did not change the plasma retinol or retinoic acid levels. Nevertheless, 9-cis β-carotene significantly inhibited atherogenesis compared to the control mice (39% reduction).

Conclusions: The results suggest that 9-cis β-carotene should be considered as an anti-atherogenic agent in the human diet.

Key words: Atherosclerosis, Dunaliella, 9CBC, LDLR-/- mice 

 


Full Text: [Abstract] [Full Article]

DOI: 10.31989/ffhd.v5i2.172

Refbacks

  • There are currently no refbacks.


Copyright (c) 2015 Noa Zolberg Relevy, Ralph Rühl, Ayelet Harari, Itamar Grosskopf, Iris Barshack, Ami Ben-Amotz, Uri Nir, Hugo Gottlieb, Yehuda Kamari, Dror Harats, Aviv Shaish

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
x
Message