Regetting-18 complex powder (R-18CP) - A formulation of components from food-medicine and its anti-aging effects
DOI:
https://doi.org/10.31989/ffhd.v15i7.1647Abstract
Background: Aging is a natural process that affects all living beings. This process leads to various physiological and pathological changes. Human health has traditionally relied on a combination of dietary supplements and herbal remedies (food-medicine homogeneity) to combat the effects of aging. Recent scientific advancements have provided more profound insights into the mechanisms by which these traditional substances contribute to the anti-aging impacts through their active components.
Objectives: A formulation called Regetting-18 Complex Powder (R-18CP) was investigated using multiple aging markers that were hypothesized to inhibit cellular senescence. This formulation included several bioactive ingredients known for their anti-aging properties, such as Panax ginseng, Ganoderma lucidum, and Polygonati rhizome.
Methods: Adult male and female zebrafish were paired overnight, and embryos were collected the following day. Six experimental groups were established, each consisting of 10 fish, and all groups were conducted in triplicate. The groups included: (1) Control, (2) Model, (3) Positive control (treated with 5 μg/mL resveratrol), and (4) three R-18CP treatment groups (treated with 1 μg/mL, 0.5 μg/mL, and 0.1 μg/mL, respectively). At 72 hours post-fertilization, various assays were performed: 10 fish per group were stained for β-galactosidase, another 10 for reactive oxygen species (ROS), 10 were analyzed for mitochondrial membrane potential, 50 were used to assess telomerase levels, and DNA was extracted from 30 fish for telomere length analysis.
Results: R-18CP reduced β-galactosidase staining intensity in a dose-dependent manner. Increasing concentrations of R-18CP were associated with decreased ROS staining intensity, elevated telomerase levels, and longer telomeres. Additionally, the mitochondrial membrane potential staining intensity increased in proportion to the R-18CP concentration.
Conclusion: Based on the five distinct aging markers assessed in the zebrafish model, R-18CP may be able to restrict aging. Further studies using human models are recommended and necessary.
Novelty of the Study: This study is the first to report that an R-18CP can exert anti-aging effects through telomere protection and improved mitochondrial function in zebrafish models. This suggests R-18CP is a potential therapeutic compound for anti-aging.
Keywords: anti-aging, food-medicine homogeneity, zebrafish, β-galactosidase, telomere, telomerase, reactive oxygen, membrane potential
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