Proline Rich Peptide-Linked Albumin Particles in Experimental Oxidative Brain Damage Model

作者

  • Kristine Danielyan
  • Nazeli Zaqaryan
  • Rita Khallouf
  • Maria Kojoyan
  • Kristine Tspnetsyan
  • Arpi Manukyan
  • Astghik Tsokolakyan
  • Mkrtich Yeranosyan
  • Samvel Chailyan

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https://doi.org/10.31989/bchd.v8i12.1835

摘要

Background: The leading contributors to death and disability are cardiovascular diseases (CVDs) and stroke. PRP-1 (Proline Rich Peptide) is a peptideisolated from the bovine hypothalamus and identified in the human brain. It was shown that this peptide may have antioxidant and neuroprotective properties. However, the circulation time of peptides in the bloodstream is limited; therefore, coupling PRP-1 with albumin nanoparticles might prolong its stability and bioavailability. The purpose of the study was to evaluate PRP-1 conjugated with albumin nanoparticles in an experimental brain injury model induced by hydrogen peroxide.

Methods: We used Transmission Electron Microscopy (TEM; Philips CM10) and Dynamic Light Scattering (DLS) to evaluate particle size and zeta potential. The brain injury model was created using hydrogen peroxide as a source of free radicals, which induced disruption of the blood–brain barrier (BBB). BBB disruption was quantified by measuring Evans Blue dye accumulation in the brain parenchyma.

Results: Through sedimentation and filtration procedures, we obtained stable nanoparticles (NPs). PRP-1–coupled NPs degraded more slowly in the presence of trypsin than control NPs. Furthermore, PRP-1/NPs partially prevented BBB disruption in the experimental model.

Conclusion: PRP-1/albumin NPs protected the BBB under experimental brain injury conditions.

Keywords: PRP-1, neurohormone, experimental stroke, albumin, nanoparticles, blood–brain barrier

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已出版

2025-12-02

期次

卷 8 期 12 (2025): December 2025

栏目

Research Articles

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