Optimal dose of Moringa oleifera Lam for hematopoietic recovery in aluminum-induced hematotoxicity
DOI:
https://doi.org/10.31989/bchd.v9i3.1944Abstract
Background: Aluminum-induced hematotoxicity disrupts hematopoiesis and leads to anemia primarily through oxidative stress mechanisms. Natural antioxidants such as Moringa oleifera have shown potential protective effects; however, the optimal therapeutic dose remains unclear.
Objective: This study aimed to evaluate the dose-dependent effects of Moringa oleifera leaf extract and determine the optimal dose to improve hematological parameters in aluminum-induced hematotoxicity.
Methods: Twenty-four male Wistar rats were randomly assigned into four groups (n = 6): control, AlCl₃ + 200 mg/kg Moringa oleifera, AlCl₃ + 100 mg/kg Moringa oleifera, and Moringa oleifera (200 mg/kg) only. Hematological parameters, including hemoglobin concentration, red blood cell count, hematocrit, and differential leukocyte counts, were measured over 14 days. Statistical analysis was performed using one-way ANOVA, with p ≤ 0.05 considered significant.
Results: Treatment with Moringa oleifera significantly improved hematological parameters compared to the AlCl₃-exposed group (p ≤ 0.05). The 100 mg/kg dose demonstrated superior efficacy, with higher hemoglobin (12.00 g/dL), red blood cell count (7.23 × 10⁶/µL), and hematocrit (41.50%) compared to the 200 mg/kg dose. Additionally, an increase in lymphocyte percentage was observed. The dose-response relationship indicated a hormetic effect, where moderate dosing produced greater benefits than higher dosing.
Conclusion: Moringa oleifera exhibits significant protective effects against aluminum-induced hematotoxicity, with 100 mg/kg identified as the most effective dose. These findings highlight its potential as a natural therapeutic agent for improving hematological health under toxic conditions
Novelty of the study: This study identifies an optimal dose (100 mg/kg) of Moringa oleifera for hematological recovery in aluminum-induced toxicity and provides evidence of a hormetic dose-response effect in hematopoietic protection.
Keywords: Aluminum chloride; anemia; dose–response; erythropoiesis; Moringa oleifera; hematopoietic recovery; bioactive dose.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Rafiah Awad Mohamed Adam, Azhari A. Mohammed Nour, Ream Hassan Ahmed, Hanan Elamin Ibrahim, Mohamad Awad Elkarim Mohamad Ibrahim, Mohammed A. Shanwaz, Wisal A. Babiker, Ibrahim Elhag Elmahdi, Soltan J. Algamdi, Abdulmajeed AA Sindi, Raed A Alharbi, Hanan E Alyahyawi, Hanan E Alyahyawi, Abdulmohsen M Alruwetei
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain the copyright of their articles and grant the Functional Food Center (FFC) and its journals the right of first publication under the terms of the Creative Commons Attribution 4.0 International License.
This license permits unrestricted use, distribution, and reproduction in any medium, including commercial use, provided the original author(s) and source are properly credited. Authors may post and share their published work freely, provided that the original publication in this journal is acknowledged.
By submitting to this journal, authors confirm that their manuscripts are original, not under consideration elsewhere, and that they hold the necessary rights to grant this license. The Functional Food Center encourages open scientific exchange and allows derivative and extended works, provided attribution to the original publication is maintained.